![]() Samples were snap frozen, and serial 5-μm-thick sections were cut. Immunohistochemistry and Immunofluorescence. See Table for clinicopathological parameters of each case, including age, tumor histological grade, tumor size, lymph node involvement, lymphatic and vascular invasion, estrogen and progesterone receptor status, proliferation index using Ki67 antibody MIB-1, DNA ploidy, and S phase. Patients' ages at the time of diagnosis ranged from 23 to 75 yr. Approximately one-half of the cases presented with metastases to regional lymph nodes. ![]() The 32 studied cases of breast carcinoma, graded according to Bloom and Richardson, were distributed as follows: ductal carcinoma, high grade III (DC III), n = 17 ductal carcinoma, intermediate grade II (DC II), n = 9 ductal carcinoma, low grade I (DC I), n = 1 mixed lobular carcinoma, n = 3 carcinoma in situ, n = 2. The material was collected under the auspices of Institutional Review Board protocol 97-79. Nine normal breast specimens were processed in the same way. All tumor samples were received as coded specimens, and for patient confidentiality, numbers corresponding to the order received in the laboratory were assigned. Tissue samples obtained at surgery were immediately embedded in OCT and then frozen and stored at −80☌ until further processed. In some cases, T cells are clustering around the mature DCs in peritumoral areas, thus resembling the DC–T cell clusters of secondary lymphoid organs, which are characteristic of ongoing immune reactions. Confirming the immature/mature DC compartmentalization pattern, in vitro–generated immature DCs adhere to the tumor cells, whereas mature DCs adhere selectively to peritumoral areas. The high numbers of immature DCs found in the tumor may be best explained by high levels of macrophage inflammatory protein 3α expression by virtually all tumor cells. We demonstrate two levels of heterogeneity of DCs infiltrating breast carcinoma tissue: (a) immature CD1a + DCs, mostly of the Langerhans cell type (Langerin +), were retained within the tumor bed in 32/32 samples and (b) mature DCs, CD83 +DC-Lamp +, present in 20/32 samples, are confined to peritumoral areas. Breast carcinoma cells were defined by morphology and/or cytokeratin expression. Mature DCs were defined by expression of CD83 and DC-Lamp. Immature DCs were defined by expression of CD1a-, Langerin-, and intracellular major histocompatibility complex class II–rich vesicles. We have analyzed the presence of immature and mature dendritic cells (DCs) within adenocarcinoma of the breast using immunohistochemistry.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |